Simultaneous determination of try acid in pretreated human blood
نویسنده
چکیده
Optimization of sample pre-treatment. Complete removal of cellular and further solid sample constituents, precipitation and removal of proteins, precipitation and removal of cholesterol from the blood sample and quantitative extraction of the analytes tryptophan, phenol, p-cresol and cholic acid in the diluted blood plasma phase. Step III: Combination and conversion of both isocratic HPLC/DAD/MS methods into a gradient HPLC/DAD/MS method for the identification and separation of the analytes tryptophan, phenol, p-cresol and cholic acid
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